Characteristics:
Source HEK293.
Description Human IGF-II(Ala25-Glu91) Accession # P01344.1
Predicted molecular 7.5kDa
Mass
Specification:
Appearance White powder,Colorless clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under conditions, visualized by Coomassie staining)
Endotoxin ≤10 EU/mg by the LAL method
Activity Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells.
The ED50 for this effect is ≤50 ng/mL.
Formulation Lyophilized from a 0.22 μm filtered solution containing TFA,ACN,5% Trehalose.
Handling and Storage:
Reconstitution It is recommended to redissolve in 4mM HCl.
Shipping The product is shipped with blue ice.
Storage & Stability 36 months at -20°C to -80°C in lyophilized state6 months at -20°C to -80°C
under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after
reconstitutionUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity:
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