Recombinant Human Fibronectin

 

Recombinant Human Fibronectin
          中文名：重组人纤维连接蛋白

 

Characteristics: 

 

Source                         CHO

 

Description                   Human Fibronectin Fragment   Accession # P02751

 

Predicted molecular     62.6 kDa

Mass

Specification:

 

Appearance                  White powder, Colorless clear liquid after reconstitution

 

Purity                             ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions,

                                      visualized by Coomassie staining)

 

Endotoxin                     ≤10 EU/mg by the LAL method

 

Activity                          Measured by the ability of the immobilized protein to support the adhesion of

                                      B16-F1 mouse melanoma cells. The ED50 for this effect is 35.0-420 ng/mL.

 

Formulation                   Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol

                                      and 0.01% Tween 80, pH 7.4

 

Size                               50 μg/vial, 1 mg/vial

 

Handling and Storage:

 

Reconstitution               It is recommended to redissolve in sterile deionized water.

 

Shipping                        The product is shipped with blue ice.

 

Storage & Stability        36 months at -20°C to -80°C in lyophilized state

                                      6 months at -20°C to -80°C under sterile conditions after reconstitution

                                      7-10 days at 2°C to 8°C under sterile conditions after reconstitution

                                      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

Bioactivity:

 

  
 

Recombinant   Human   Fibronectin   (Cat. No.   mly01301) stimulates adhesion of B16-F1 cells. The ED50  for this effect is 35.0-420 ng/mL The activity of mly01301 was higher than other competing products.