Recombinant Fish bFGF

Recombinant Fish bFGF
         中文名：重组鱼bFGF

 

Characteristics: 

  

Source                         E. coli

 

Description                  Fish FGF2 (M1-C155)Accession # XP_030252529.1

 

Predicted molecular    17. 1kDa

Mass

Specification:

 

Appearance                White powder,Colorless clear liquid after reconstitution

 

Purity                          ≥95%, by SDS-PAGE (under reducing (R)&Non-reducing conditions,visualizedby Coomassie staining)

 

Endotoxin                   ≤10 EU/mg by the LAL method

 

Activity                        Measured in a cell proliferation assay using NIH3T3 mouse embryonic
fibroblast cells. The ED50 for this effect is    ≤2.0 ng/mL.

 

Formulation                 Lyophilized from a 0.22 μm filtered solution containing 10 mM PB, 250 mMNaCl, 5% mannitol and 0.01% 
Tween 80, pH7.4

 

Size                             50 μg/vial, 1 mg/vial

 

Handling and Storage:

 
Reconstitution              It is recommended to redissolve in sterile deionized water.

Shipping                       The product is shipped with blue ice

 

Storage & Stability        36 months at -20°C to -80°C in lyophilized state
                                      6 months at -20°C to -80°C under sterile conditions after reconstitution
                                      7-10 days at 2°C to 8°C under sterile conditions after reconstitution
                                      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Bioactivity: 


 

Recombinant Fish bFGF (Cat. No. mly00341) stimulates proliferation of NIH3T3 cells. The ED50 for this effect is  ≤2.0 ng/mL. The activity of recombinant Fish bFGF (Cat. No. mly00341) was higher than the other competing product.