Characteristics:
Source Chinese Hamster Ovary cell line
Description Human IL-18 (Tyr37-Asp193)
Accession # Q14116
Predicted molecular 18.2 kDa
Mass
Specification:
Appearance White powder, Colorless clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions,
visualized by Coomassie staining)
Endotoxin ≤10 EU/mg by the LAL method
Activity Measured by its ability to induce IFN-gamma secretion by KG-1 human
acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 .
for this effect is ≤2.0 ng/mL.
Formulation Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol
and 0.01% Tween 80, pH 7.4
Size 50 μg/vial, 1 mg/vial
Handling and Storage:
Reconstitution It is recommended to redissolve in sterile deionized water.
Shipping The product is shipped with blue ice.
Storage & Stability 36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity:
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Measured by its ability to induce IFN-gamma secrection by KG-1 cells in the presence of TNF-alpha.
The ED50 for this effect is ≤2.0 ng/mL. The activity of Recombinant Human IL-18 ( Cat. No. mly02821H ) is equivalent to other competing products.
