Recombinant Human - Fibronectin

Recombinant Human - Fibronectin
          中文名：重组人纤维连接蛋白

 

Characteristics: 

 

Source                            E.coli

 

Description                     Human Fibronectin Fragment

                                       Accession # P02751

 

Predicted molecular       62.6 kDa

Mass

Specification:

 

Appearance                    White powder, Colorless clear liquid after reconstitution

 

Purity                              ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions,

                                       visualized by Coomassie staining)

 

Endotoxin                       ≤10 EU/mg by the LAL method

 

Activity                            Measured by the ability of the immobilized protein to support the adhesion of

                                       NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is
     35.0-420 ng/mL.

 

Formulation                    Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol

                                       and 0.01% Tween 80, pH 7.4

 

Size                                50 μg/vial, 1 mg/vial

 

Handling and Storage:

 

Reconstitution                 It is recommended to redissolve in sterile deionized water

 

Shipping                          The product is shipped with blue ice.

 

Storage & Stability           36 months at -20°C to -80°C in lyophilized state

                                         6 months at -20°C to -80°C under sterile conditions after reconstitution
                                         7-10 days at 2°C to 8°C under sterile conditions after reconstitution

                                         Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

Bioactivity: