Recombinant Human GH

Recombinant Human GH
          中文名：重组人GH

Characteristics: 

 

Source                            Chinese Hamster Ovary cell line

 

Description                      Human GH (Phe27-Phe217)   Accession # P01241

 

Predicted molecular        22 kDa

Mass

Specification:

 

Appearance                      White powder,Colorless clear liquid after reconstitution

 

Purity                                ≥95%, by SDS-PAGE (under reducing (R)&Non-reducing

                                         conditions,visualized by Coomassie staining)

 

Endotoxin                         ≤10 EU/mg by the LAL method

 

Activity                             Determined by its ability to stimulate the proliferation of Nb2-11 cells.

                                         Expected ED50 ≤ 0.1 ng/ml, corresponding to a specific activity of  ≥  1 x 10
     7 units/mg

 

Formulation                      Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol

                                         and 0.01% Tween 80, pH 7.4

 

Size                                  Lyophilized from a 0.22 um filtered solution containing 10mM PB

 

Handling and Storage:

 

Reconstitution                   It is recommended to redissolve in sterile deionized water.

 

Shipping                            The product is shipped with blue ice

 

Storage & Stability           36 months at -20°C to -80°C in lyophilized state

                                          6 months at -20°C to -80°C under sterile conditions after reconstitution
                                          7-10 days at 2°C to 8°C under sterile conditions after reconstitution

                                          Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

Bioactivity:

 

 

Measured in a cell proliferation assay using Nb2-11 cells.