Characteristics:
Source Chinese Hamster Ovary cell line
Description Human FGF4(Ala31-Leu206) Accession # P08620
Predicted molecular 19.3 kDa
Mass
Specification:
Appearance White powder, Colorless clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin ≤10 EU/mg by the LAL method
Activity Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED50 for this
effect is 0.25-1 ng/mL
effect is 0.25-1 ng/mL
Formulation Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
Size 50 μg/vial, 1 mg/vial
Handling and Storage:
Reconstitution It is recommended to redissolve in sterile deionized water.
Shipping The product is shipped with blue ice.
Storage & Stability 36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity:
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Recombinant Human FGF4 (Cat. No. mly00901) stimulates proliferation of NIH3T3 cells. The ED50 for this effect is 0.25-1ng/mL. The activity of mly00901 was higher than the other competing product.
