中文名：重组人表皮生长因子 EGF
Characteristics:
Source Chinese Hamster Ovary cell line.
Description Human EGF (Asn971-Arg1023) Accession # P01133
Predicted molecular 6.2 kDa
Mass
Specification:
Appearance White powder, Colorless clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions,visualized by Coomassie staining)
Endotoxin ≤10 EU/mg by the LAL method
Activity Measured in a cell proliferation assay using NIH3T3 mouse embryonicfibroblast Cells. The ED50 for
this effect ≤0.3 ng/mL.
Formulation Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitoland 0.01% Tween 80, pH 7.4.
Size 50 μg/vial, 1 mg/vial
Handling and Storage:
Reconstitution It is recommended to redissolve in sterile deionized water.
Shipping The product is shipped with blue ice.
Storage & Stability 36 months at -20°C to -80°C in lyophilized state6 months at -20°C to -80°C under sterile
conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitutionUse a
manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity:
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Recombinant Human EGF (Cat. No. mly00801) stimulates proliferation of NIH3T3 cells. The ED50 for this effect ≤0.3ng/mL. The acivity of mly00801 was equivalent to other competing products.
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Measured in a cell proliferation assay using NIH3T3 cells.
