Characteristics:
Source CHO
Source CHO
Description Human Wnt-3a (Ser19-Lys352) with a C-terminal linker GGGGGS and Human lgG1
(Asp104-Lys330)Accession # NP_149122
Predicted molecular 58.2kDa
mass
Specification:
Appearance White powder,Colorless clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under reducing (R)&Non-reducing
conditions,visualized by Coomassie staining)
Endotoxin ≤100 EU/mg by the LAL method
Activity Measured by its ability to induce Topflash reporter activity in CHO-K1 cells.
The ED50 for this effect is ＜30ng/ml.
Formulation Lyophilized from a 0.22 um filtered solution containing PBS, pH7.4
Size 50 μg/vial, 1 mg/vial
Handling and Storage:
Reconstitution It is recommended to redissolve in sterile deionized water.
Shipping The product is shipped with blue ice
Storage & Stability 36 months at -20°C to -80°C in lyophilized state 6 months at -20°C to -80°C under sterile conditions
after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity:
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Measured by its ability to induce Topflash reporter activity in CHO cells in the presence of 5ng/ml BMP2. The ED50 for this effect is ＜ 30ng/ml. The activity of Recombinant Human Wnt-3a(hFc Tag ） (cat no mly04603) was higher than other competing products.
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