Characteristics:
Source Chinese Hamster Ovary cell line
Source Chinese Hamster Ovary cell line
Description Human IFNγ (Gln24-Gln166) Accession # P01579
Predicted molecular 16.8 kDa
mass
Specification:
Appearance White powder, Colorless clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions,
visualized by Coomassie staining)
Endotoxin ≤10 EU/mg by the LAL method
Activity Measured in a cell proliferation assay using Mo7e human leukemia cell line. The ED50 for this
effect is ≤ 2 ng/mL,corresponding to a specific activity of ≥ 2 x 107 units/mg.
effect is ≤ 2 ng/mL,corresponding to a specific activity of ≥ 2 x 107 units/mg.
Formulation Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
Size 50 μg/vial, 1 mg/vial
Handling and Storage:
Reconstitution It is recommended to redissolve in sterile deionized water.
Shipping The product is shipped with blue ice.
Storage & Stability 36 months at -20°C to -80°C in lyophilized state 6 months at -20°C to -80°C under sterile conditions
after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution
after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity:
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Recombinant Human IFNγ (Cat. No. mly01801) stimulates proliferation of Mo7e cells. The ED50 for this effect is ≤2 ng/mL. The activity of mly01801 was higher than the other competing product.
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