Characteristics:
Source Chinese Hamster Ovary cell line
Description Human Activin A (Gly311-Ser426) Accession # P08476
Predicted molecular 12.9 kDa
Mass
Specification:
Appearance White powder, Colorless clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin ≤10 EU/mg by the LAL method
Activity Lyophilized from a 0.22 μm-filtered solution containing 0.085% TFA,30%ACN, 5% mannitol, pH 2.5
Formulation Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
Size 50 μg/vial, 1 mg/vial
Handling and Storage:
Reconstitution It is recommended to redissolve in 4mM HCl.
Shipping The product is shipped with blue ice.
Storage & Stability 36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity:
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Determined by its ability to inhibit the proliferation of mouse MPC-11 cells. The expected ED50 is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.
