Characteristics:
Source Chinese Hamster Ovary cell line
Description Human TGF-β1 (Ala279-Ser390) Accession # P01137
Predicted molecular 12.8 kDa(monomer)
Mass
Specification:
Appearance White powder, Colorless clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin ≤10 EU/mg by the LAL method
Activity Measured in a cell inhibition assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0. 1ng/mL.
Formulation Lyophilized from a 0.22 μm-filtered solution containing 0.085% TFA, 30% ACN, 5% mannitol, pH 2.5
Size 50 μg/vial, 1 mg/vial
Handling and Storage:
Reconstitution It is recommended to redissolve in 4mM HCL.
Shipping The product is shipped with blue ice.
Storage & Stability 36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity:
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Measured in a cell inhibition assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0. 1ng/mL. The ED50 for this effect is ＜0.5 ng/mL. The activity of mly04201N N was higher than other competing products.
