Characteristics:
Source Chinese Hamster Ovary cell line
Source Chinese Hamster Ovary cell line
Description Human IFN-α2b (Cys24-Glu188) Accession # P01563
Predicted molecular 19.2 kDa
mass
Specification:
Appearance White powder,Colorless clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under reducing (R)&Non-reducing
conditions,visualized by Coomassie staining)
Endotoxin ≤10 EU/mg by the LAL method
Activity Measured in a VSV inhibition assay with Wish cells. The ED50 this effect is
≤100 pg/mL, corresponding to a specific activity is ≥2 × 108 units/mg.
Formulation Lyophilized from a 0.22 um filtered solution containing PBS,5% mannitol and 0.01% Tween 80, pH7.4
Handling and Storage:
Reconstitution It is recommended to redissolve in sterile deionized water.
Shipping The product is shipped with blue ice
Storage & Stability 36 months at -20°C to -80°C in lyophilized state 6 months at -20°C to -80°C under sterile conditions
after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution
after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity:
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Measured in a VSV inhibition assay with Wish cells. The ED50 this effect is ≤100 pg/mL, corresponding to a specific activity is ≥2 × 108 units/mg. The activity of mly06301 was higher than the other competing product.
