Recombinant Human GDNF(E.coli-expressed)

Characteristics: 

 

Source                            E.coli

 

Description                      Human GDNF(S78-I211 ) 
 Accession #NP_000505.1

 

Predicted molecular        15.2 kDa

Mass

Specification:

 

Appearance                   White powder,colorless clear liquid after reconstitution

 

Purity                             ≥95%, by SDS-PAGE (under reducing (R)& Non-reducing conditions,

                                      visualized by Coomassie staining)

 

Endotoxin                       ≤10 EU/mg by the LAL method

Activity                           Measured in a cell proliferation assay using SH-SY5Y humanneuroblastoma cells. The ED50 for 
                                      this effect is  ≤20 ng/mL in the presenceof Recombinant Human GDNF R alpha -1 .
                                      
Formulation                     Lyophilized from a 0.22 um-filtered solution containing 20 mM Sodium 
                                        Citrate, ,5% Mannitol, pH 2.5

 Size                                10 μg/vial, 50 μg/vial, 1 mg/vial

Handling and Storage:

 Reconstitution                   It is recommended to redissolve in sterile deionized water.

Shipping                             The product is shipped with blue ice

 

Storage & Stability             36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution 
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.