Recombinant Human PDGF-BB

Characteristics: 

 

Source                                Pichia pastoris

 

Description                         Human PDGF-BB (Ser82-Thr190) 
      Accession # P01127

 

Predicted molecular            12.2 kDa

Mass

Specification:

 

Appearance                        White powder, Colorless clear liquid after reconstitution

 

Purity                                  ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions,visualized by Coomassie staining)

                                  

Endotoxin                           ≤10 EU/mg by the LAL method

Activity                                Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. 
                                           The ED50 for this effect is  ≤5 ng/mL.
                             
Formulation                       Lyophilized from a 0.22 μm-filtered solution containing 100mM Citrate-sodium 
                                          citrate, 5% Mannitol 0.1% Trehalose pH 6.5

Size                                   1 mg/vial

Handling and Storage:

 Reconstitution                   It is recommended to redissolve in sterile deionized water.

Shipping                            The product is shipped with blue ice.

 

Storage & Stability             36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution 
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

                         

 Bioactivity:

 

 

Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED50  for this effect is ≤5 ng/mL. The activity of  mly03401  is equivalent to the other competing product.