产品货号 : mlR19406
英文名称 : PRPK
中文名称 : TP53调节激酶抗体
别 名 : C20orf64; Nori 2; Nori 2p; Nori-2; p53 related protein kinase; p53-related protein kinase; PRPK_HUMAN; TP53 regulating kinase; TP53-regulating kinase; TP53RK.
研究领域 : 细胞生物 信号转导 激酶和磷酸酶
抗体来源 : Rabbit
克隆类型 : Polyclonal
交叉反应 : Human, Mouse, Rat, Dog,
产品应用 : ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 : 28kDa
细胞定位 : 细胞核
性 状 : Lyophilized or Liquid
浓 度 : 1mg/ml
免 疫 原 : KLH conjugated synthetic peptide derived from human PRPK:81-180/253
亚 型 : IgG
纯化方法 : affinity purified by Protein A
储 存 液 : 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 : Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed : PubMed
产品介绍 : PRPK is a 253 amino acid protein kinase that phosphorylates Ser15 of p53. PRPK phosphorylation of p53 causes increased stabilization and activity of p53. CGI-121 may act as an inhibitor of the PRPK-p53 interaction, thus preventing the phosphorylation of p53. Unphosphorylated p53 is degraded by the ubiquitin-proteasome pathway, which may ultimately lead to cell proliferation. PRPK contains a protein kinase domain with a conserved catalytic core. PRPK is localized to the nucleus of the cell and is highly expressed in testis, with lower expression in heart, kidney and spleen.
Function:
Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP (By similarity). Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation.
Subcellular Location:
Nucleus.
Tissue Specificity:
Highly expressed in testis. Weakly expressed in heart kidney and spleen.
Similarity:
Belongs to the protein kinase superfamily.
Contains 1 protein kinase domain.
SWISS:
Q96S44
Gene ID:
112858
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
