Recombinant Human VEGF165
中文名：重组人VEGF165
Characteristics:
Source Chinese Hamster Ovary cell line
Description Human VEGF165 (Ala27-Arg191) Accession # P15692
Predicted molecular 19. 1kDa
Mass
Specification:
Appearance White powder, Colorless clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin ≤10 EU/mg by the LAL method
Activity Measured in a cell proliferation assay using HUVEC human
umbilicveinendothelial cells. The ED50 for this effect is 0.2-5 ng/mL.
Formulation Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
Size 50 μg/vial, 1 mg/vial
Handling and Storage:
Reconstitution It is recommended to redissolve in sterile deionized water.
Shipping The product is shipped with blue ice.
Storage & Stability 36 months at -20°C to -80°C in lyophilized state6 months at -20°C to -80°C under sterile
conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after
reconstitutionUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity:
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Recombinant Human VEGF165 (Cat. No. mly04701) stimulates proliferation of HUVEC human
Recombinant Human VEGF165 (Cat. No. mly04701) stimulates proliferation of HUVEC human
umbilicvein endothelial cells. The ED50 for this effect is 0.2-10 ng/mL. mly04701 was higher than the other competing product.
